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Last Chance to Save on this Super Popular Product!!!

OK today’s Flash Deal is really a Super Limited One because we only have a finite amount of stock left.

You may recall that for years I was a big fan of TA-65.  Actually I still am! Unfortunately I cannot get along with the individuals in charge of the company and they will no longer sell me any product null and voiding my distribution contract.

But I still have some LOW Dose TA-65 30 count bottles left and I am selling them for the incredible low price of 3 for $289.95 and I am throwing a 4th bottle in Free when you buy those three! You get 4 bottles for less then the normal price of 3!

This is a one time 48 hour deal so take advantage of it because when its gone I will never be selling TA-65 again.

Ever!!!!

Supplies are limited and its first come first serve so when we run out I cannot honor any “Sorry I missed your sale but I still want the product” requests.

This one was not my fault but I took an incredible amount of abuse for it.

You however, still benefit so Act Now!!

 

Best of the Holidays!

Doc

 

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New Cancer Pathway Involves Telomeres

Drum roll and trumpets please!  A new cancer causing pathway has been found. It involves telomeres of course.

I bring it to your attention though, not for what it has shown us, but for how it its marketed. You see even scientists know they stand a better chance of getting recognition if they blow the trumpets and role the drums.  That means press releases and social media nonsense that is written by non scientists.

Now don’t misunderstand me. I give them the same “get out of jail pass” I often claim: “I didn’t make this world, I just live in it!”

Still as your source of the truth about all things telomere related, especially when it comes to your health and your understanding, I consider it my calling in life to make sure you are not blown all over the map by media hype and internet nonsense.

So let’s go over a few facts:

1)      Fact one: Telomerase does NOT CAUSE CANCER. Every time I write this I hope it is my last but that is never the case it seems.  85% of human cancers turn on telomerase somewhere in the process of becoming cancer. In the one or two cases we are clear on its actually one of the last steps ( villous adenoma to colon cancer) but cancer genetics are a diverse thing and no one knows the exact sequence that mutations happen in all cancers. There is a growing contingent of people out there who feel that contrary to 65 years of research, cancer may not even be a genetic disease. When telomerase is expressed in cancer it can be a) mutated, b) amplified c) transposed d) any other genetic abnormality that can lead to over expression! The key is to understand that things happen to cause cancer long before telomerase expression shows up to immortalize the cells, and that those things lead to the loss of normal control of many genes and their end products. Telomerase over expression does not seem to cause cancer by itself in the absence of other major changes.

2)      Telomerase is required for ultra long longevity and gene integrity in cells. Two of the best examples are germ cells (the things that become sperm and eggs) where there is a large amount of telomerase expression and stem cells where there is less. The later fact is probably one of the main reasons stem cells age albeit at a slower rate than the rest of our cells.

3)      There are normal “cell cycle check point” mechanisms that have been known for some time now that need to be bypassed in order for cancer to take hold.

4)      Longer telomeres are associated with a cleaner genome (fewer mutations) cleaner mitochondrial cell powerhouses (fewer mutations better function and better ability to make new mitochondria) and a lower and less sever incidence of cancer.

5)      While most cancers turn on telomerase there is another way for cancer cells to lengthen their telomeres and survive that does not involve telomerase.  This generally is reserved for the badest of the bad in terms of genetic instability (actually if I am being scientifically correct its “genomic instability” but you know what I mean!). This mechanism is called ALT.

6)      The ability to divide endlessly either using telomerase or ALT is one of the hallmarks of human cancer.

That brings me to the “latest findings”.

One Dr Jan Karlseder of the Salk institute discovered one of the mechanisms behind the ALT method of lengthening telomeres. Dr Karlseder found a protein called ASF-1 that drops down to zero and triggers cells in culture to use the ALT mechanism to lengthen their telomeres.

All well and good but here are my gripes.

Cancers in real life have measurable levels of that protein. It does not drop to zero in ALT driven cancers.

The super irritating insinuation that I see in all such articles “This will help us develop drugs to fight cancer”  is present here. Cancer drugs are expensive, Cancer drugs are deadly, Cancer drugs divert research away from, gasp, cancer prevention which no scientists is willing to admit they believe in. And there is no money in prevention. If you think this is a joke mark my words a cancer preventative treatment that is not a patentable drug will not be released until a drug company owns it. At least not over their dead bodies. That said most of the quacks who claim to have cured cancer and have been jailed for such statements actually do belong there!

Next, the press release is entitled “Cancer Causing Pathway Explained”.

Now I bet this was the sole concoction of the press release company. I do not think that such smart scientists would allow their discovery to be called named this way. The ALT pathway does not cause cancer, it is probably one of the last or later pieces in the puzzle.  By naming it this way they are propogating the myth that telomeres are responsible for cancer.

Next there is the usual talk of “balancing cell aging with risk of cancer” . This implies that we get old and die to prevent cancer from killing us. After I am done with the LOL ing I want to remind everyone that old cells, senescent cells seem to excrete inflammatory and yes cancer causing signals. For this reason it seems nonsensical to propose that getting old protects us from cancer. Especially when cancer is a disease of aging in 95% of all cases!

Summing it up, I would say it this way: We are barking up the wrong tree. Telomerase and telomere lengthening are cancer protective under normal circumstances. Guys you have to keep looking for the chicken that is laying the egg not examining the ingredients in the omelet!   This is also the reason I am intrigued if not convinced of the metabolic cause/component of cancer.  The genetic stuff leads nowhere but to an ever increasingly large ‘room full of pathways’ that has no end. Great for researchers and crappy for people who have or want to prevent getting cancer.

In 12+ years of research I can tell you two things with surety. Walk around with “high” omega 3 levels and keep your telomeres long anyway you can.

There are quite a number of famous scientists who are doing just that even though they will not say it in front of their peers for fear of ridicule. Me? I don’t care who ridicules me. I refuse to sit on my ass and get old when there are real answers to slowing and eventually stopping the process.

I will make this prediction. Classic science just like classic medicine will not make major breakthroughs in aging and health span. They will trumpet their findings just like the article above but the real breakthroughs will come from people who are actively seeking the answers and doing something about it every day .

That is you and me my friend!

Best,

Dr Dave

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TTAGGG You’re It! Stress, Disease and Telomere Length

The repeating sequence of the Telomere-TTAGGG has proven to be a gold mine for determining biologic age ( how old your body is acting!) and disease prognosis ( how bad you are likely to get something) as well as the effects of stress.

A recent study on the effects of racism on the telomere length of black men was the latest to show how social stress can affect longevity.  I have concerns about how the study was done but I think it makes sense to say that this kind of stress is detrimental to anyone’s health no matter what race or color.

This study builds on previous studies done in orphans who grew up with universally shorter telomeres than non orphans,  and the effects of stress on caregivers responsible for sick children.  All of these situations lead to more rapid aging and faster decline.  You can bet being sick in general can shorten your telomeres and your life.

This was shown by another study involving men between 50 and 75 who were admitted with ACS, acute coronary syndrome. Basically they were on their way to having a heart attack and no doubt some of them did.  The ones with the shortest telomeres did worse.

This study echoes the now famous Farezeneh Far, Blackburn study that showed that Omega 3 (fish oil) levels were protective of telomeres in heart disease.

So it seems everyone is linking just about everything bad that can happen to you to short telomeres and everything good to long telomeres.

It would be a good time for me to mention that other diseases of aging like Cancer and Alzheimer Disease ( yes they dropped the ‘s!) are also linked to telomere length in terms of incidence and severity.

Now there is still a lot of disagreement among researching scientists as to whether telomere loss is actually all that important in aging.  This pretty much depends on what their pet project is and where their grant money comes from.

I have a simple ( my PhD colleagues would say simplistic!) way of explaining this. My friend Maria Blasco did a study recently that showed that turning on telomerase extends life span in adult and old adult mice.  That was the end point of the study.  There are many other people out there looking at things like intracellular junk (lipofuscin) mitochondrial function, calorie restriction, sirtuin proteins and mTOR inhibitors.  None of these strategies have been shown by themselves to lengthen life although some of them seem to improve health.

I am certain that ALL of these things were improved by telomerase activation or the mice would not have lived longer.  You can’t live longer with declining mitochondria, abnormal proteins and increasing intracellular junk.  It’s simply not possible.  But because these things were not specifically measured common sense takes a back seat to scientific skepticism.

Here’s the thing: They would rather remain skeptical than investigate these questions probably because their grand money does not include answering these questions.  It does involve protecting their source of income for their pet project however.

Here’s another thing: as I said above Telomerase Activation seems to be the only current feasible way to extend life in mammals.  The folks spending time on all that other stuff have been unable to demonstrate increased longevity AND healthspan.

Want it to be even more simple?

I will quote that bastion of American Health who brought us the great Dr Oz.

Yes, I am talking about Oprah who said “I want long telomeres!” She should be on TA-65!

You probably should too!

I am entering year 5 with nothing but positive improvements in both telomere length and % of short telomeres. Both are going the right way for decreasing biologic age.

What is youth worth to you?

Doc

PS what I have said here is especially important if YOU are under constant or severe stress, get sick a lot or have a family history of one of those nasty diseases above!

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Dietary approaches to prevent aging

As you may know already, I wrote a book on telomeres back in 2010 called the The Immortality Edge.

While this was a ‘telomere centric’ book, it did focus on all kinds of things to help keep those biologic time clocks ticking. In spite of criticism, I maintained (and still do) that supplementation was necessary to achieve optimal levels of certain naturally occurring compounds in your body at a level that would actually impact disease and the aging process all at once.

Of course I focused on TA-65 and Omega 3 fish oils.

There have been some really idiotic releases on the internet in to the usual suspects.

One recent concept that gets repeated every so often is the “fish oil no good for heart disease” crap.

While I jokingly and repetitively say, “Fish oil no good for heart disease” on Facebook every time a positive article comes out, I thought I would show you some studies just to back up my words.

My personal favorite was a multi-center trial done in Italy, called the GISSI Prevenzione or GISSI-P for short. This one did not get a lot of play in the US but it is actually the source for a lot of great info on cardiac disease and fish oil.

What they found:

“Modest dose omega-3 fatty acid has been shown in GISSI-P (11,324 patients, follow-up 3.5 years) to produce a reduction in sudden death of 45%, and in cardiac death of 35%, acting probably via an anti-arrhythmic effect. In another study called JELIS (18,645 patients, follow-up 4.6 years), high dose omega-3 given to Japanese patients on a high fish diet and already on statin treatment produced further benefit with a 19% reduction of nonfatal cardiovascular outcomes.”

Now as you can imagine, their version of “High Dose” and mine are not the same and once again they did not do one of the most important tests you can – the Omega6/3 ratio.

But they still got some great results as you can see. One number left out above was “all-cause mortality” which basically means the number of people dying from anything.  Twenty-eight percent fewer people died when on omega 3’s.

I recently had a conversation with my friend Dr Tom Gilhooly in Scotland about the omega 3 test. Like me, Tom feels that this might be one of the most important things you can test. Tom is a lot more “violent” in bludgeoning his colleagues about the need for this than I am.  He laughed when I told him I try to work with doctors who still use statins because I know that fish oil will improve the outcomes and improve the tolerability. Tom is not a fan of statins at all and does not even bother with this approach.

Thanks to Tom, I will soon be speaking to a real legend in the field, Dr William Lands, who was really the person who looked at the Omega6/3 ratios and tied them to reduction in heart disease.

Now we also know they seem to benefit congestive heart failure, which is a very different animal than vascular heart disease (heart attack).

Why focus on heart disease?  Well it’s still the number one killer by far in Western Society and if we got people’s fish oil omega 3 levels up we could potentially prevent 250,000 deaths per year and reduce hospitalizations by a much larger number.

This would save untold billions for our health care system.

In an article called “Dietary approaches that delay age-related diseases,” fish oil was ranked against other interventions for its effectiveness based on the existing evidence a few years ago.

Some of the other interventions included calorie restriction, fruit and vegetable consumption, plant fatty acids and various vitamins including B and C.  The diseases included Age Related Macular Degeneration, Alzheimer’s, Heart disease, and more.

Taken as whole fish oil won hands down and that was thousands of studies ago.

To me Fish Oil still remains one of the most cost effective anti-aging “drugs” out there, especially when you look at the effects in GISSI-P and other studies.  No single drug comes close.

What is the lesson?

Take your fish oil and take enough to get your ratio into a good range.  Once you do you should only have to do the test once a year.

Ultra Potent Fish Oil – Omega 3 Test Kit

A small price to pay for all those wonderful things!

Doc

P.S. While I can’t say fish oil lengthens telomeres, I am sure it protects them based on studies done in the past two years, all of which I have written about in the past.

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What is your life worth

When I ask people that question you’d be surprised at the answers I get.  The dollar values go from $10,000 on the low side (no, I am not kidding) to priceless.  And of course there are the people unwilling to even play this as a game who say, “every life is priceless”.

So, I am going to ask you the question in a different way.

How often do you think of your healthspan and lifespan when you make the following choices:

 

1)      How much sleep to get on a regular basis

2)      How much exercise and what kind to do on a regular basis

3)      The food and supplement choices you make

4)      Where you get your information on health from

5)      Do you meditate regularly or not

6)      Have you had your Vitamin D or Omega 6/3 ratios tested on at least an annual basis?

Whether you know it or not, each and every one of those questions relates directly to the length of your biologic time clocks, known as telomeres. If you read the book “The Immortality Edge”, then you have a pretty strong inkling about why these are important questions and why they form the cornerstone of healthy lifestyles.

The truth is there are many sources for information about your health. If you believe that the best source is someone who sells pots, pans and magnetic widgets and just about everything under the sun, then you are missing the point.  I am not the only one with good information, but I am the only one who has written a book about what is clearly the most important biologic marker for aging ever discovered: the telomere. My old motto “others will copy but none compare” has never been truer than in this field of cutting edge, anti-aging medicine.

I have always believed everyone should do everything they can to preserve this life and to improve their health for as long as possible. I have been a guinea pig for that very purpose many times in my life. I continue to take large doses of my own ultra-pure fish oil every day and to monitor my Omega 6/3 ratios.

Next week, I will be in Iceland doing research on that very topic. I am also in my third year of taking TA-65, not only because it is helping my telomere length and because it is the only telomerase activator with real human studies, but because I feel great on it and less great when I stop it.

I understand you may not think you can afford TA-65.  All I can do is ask the question, “What is your life worth?” If the answer is “not that much!” then you should have a serious look at the Telomere Edge Packs, which I designed to fill the need for something less expensive, but still giving good support to those all important telomeres of yours.

Either way, I think you are worth it and I’d bet there is at least one other person who agrees!

Doc

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Fish oil and cancer

This week, the National Academy of Sciences released a statement, saying that Omega 3 fatty acids (which are EPA and DHA, found in fish oil) may help treat or even prevent cancer. Those are their words, not mine and given the possibility of me winding up prosecuted for even thinking such things, I want you to be clear on who is saying what!

I will tell you this is not new information.  There are reams of data, much of which I have covered for my readers, since I started broadcasting my newsletter on the internet in 2002, which have said the very same thing.

But, many doctors still do not believe.  At some point in the future, we can expect a prescription Omega 3-derived, highly modified cancer drug from Big Pharma, with an unpronounceable name, and then it will be OK for doctors to use it. It will also have to cost your insurance company thousands of dollars, or it won’t be put into use!

In the next two months, I will be talking a lot about the biologic time clocks called telomeres, the supplement that has human data to show positive effects on them, TA-65, and the beneficial combination of fish oil.

But, today I want to focus on some things that will help you understand just how important fish oil is for your health, including the whole issue of cancer.

Normally, I would not focus on a single isolated nutrient and tell you it will help your health that much. But, in the case of fish oil and telomerase activators, the areas where these work are so broadly related to health and longevity that they actually do make that big an impact, even in the absence of other things you could and should do.

Fish oil is everywhere and does a ton of different things.  It’s in your cell membranes and determines how well they work.  It’s in all the other membranes inside the cell, like the mitochondria (powerhouses) and nucleus (where your genetic material is) and it determines how well they work.  It releases anti-inflammatory mediators inside the cell and outside to other cells.  It controls free radical generation in far more potent fashion than many antioxidants. It is part of the signaling mechanism that removes sick, dead and dying cells and cells that have gone rogue, like cancer cells. It helps determine neurotransmitter levels in your brain and electrical signals in your heart and blood vessels. And there are four decades of unbiased scientific information surrounding these topics.

There are case reports of cancers disappearing using high dose (15 grams a day) of Fish oil. There are similar reports and scientific studies showing similar results in breast and prostate cancer. You should not expect these results and I am not saying you will get them. I am simply pointing out why there is sudden interest in fish oil and cancer (yet again!).

We know that fish oil up-regulates anti-cancer genes. We know that it changes methylation and other epigenetic markers to favor lower inflammation and tumor growth. We now know from this round of studies that increasing Omega 3 (EPA, DHA) and decreasing its opposite Omega 6 (vegetable oils, grains and nuts), reduces cancer cell growth differentiation and blood supply.

We also know that consumption of said fatty acids may add years to your life, as another round of recent studies and articles has shown.

We know that these effects seem to depend on the ratio of omega 6 to omega 3 fatty acids in our blood and tissues. We know that pursuing healthy levels will help us avoid heart diseases, tumors, auto immune diseases, arthritis, depression, including postpartum depression and many other maladies. But we do not test these levels when such a test is easily available.

And we do not, as a nation, get nearly enough Omega 3’s.

You too can access the best EPA/DHA fish oil: ultra-strong, weapons grade.  The best way to determine which one you need  is to do the little finger stick test finger stick test and see how good or bad you are and pick dosage from there.  Six capsules a day (2 with meals is easy!) is my recommendation to start with.

Ultra Potent Fish Oil

I am now in my 11th year of crusading for the health benefits of fish oil publicly. I used it in my practice and personal life for years before that.  I am in my 5th year crusading for TA-65 and telomerase activation.

You cannot imagine what the appropriate use of this information has done for me and thousands of others…unless you are doing it yourself!

Doc

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A Sea of Wanting, A Sea of needing

Someone asked me the other day what it’s like to speak to a large audience about staying young and healthy.

My reply startled her. I said it was like looking out over a sea of wanting and needing.

“What do you mean?” she asked.

I said, “There are so many motivated people who want to know the truth but have been deceived so many times they no longer have faith that something real can be done to improve their futures. Worse, there are always a large group of people who believe conspiracy theories and are angry at the world around them for ruining their lives and those of their loved ones. Always! Rather than focusing on what they can do to help themselves and the ones they love, they live a betrayed angry and dependent life lashing out at all the ‘mistakes’ that have been made by ‘those greedy basxxrds in power’. Problem is they are actually right at least 50% of the time – but it does not solve the problem.

And finally there is the worst case scenario – the people who are so sure they are healthy and doing the right thing they don’t bother to test.”

“Oh, c’mon doc. You lecture to other doctors; there must be some healthy ones!”

“Yes, there are – but you would be shocked to learn this crowd is one of the unhealthiest bunch of people out there. I can’t tell you how embarrassed I was for us as a profession at the last anti-aging conference I attended. So many old, tired looking, fat doctors wallowing around, looking for the magic pill just like everyone else!”

Doctors pride themselves on their scientific knowledge  but most of them don’t know a whit about keeping you younger.  I wrote an email to you quite some time ago about when I was a drug dealer that is as much as anything, an admission of that very same problem.

But then I devoted my life to fixing it.

Hence my comment about wanting and needing. Everyone wants the magic pill but for now the closest thing that is affordable for most is Fish Oil

“OK, Dr Dave; what is missing and what do we really need to do first?”

“You won’t like the answer!” I replied. “It’s not sexy, revolutionary or even all that interesting. You see, the magic pill is actually lifestyle modification – the right diet, the right kinds and amounts of exercise and proper long term sleep habits with some fish oil and a few other things thrown in.”

“Oh Puulllleeez!” she cried, “Not that same old same old again! Besides, I take fish oil every day.”

“How Much?” I queried.  “Two capsules a day most days.”

I am sure she wished she’d never started the conversation at that point because I went on to lecture her about everything you should already know if you’ve been paying attention: 6 grams minimum to get into primary cardiac prevention range, plant-based diets and supplements are lacking and don’t contain or convert to enough EPA, red meat is stigmatized because no one has looked at free range products in studies, and of course the inevitable mention of how well fish oil supports telomeres.

“So you are saying that fish oil in the correct dose is more important even than say, Vitamin D or other vitamins?” she wondered.

“Yup! Start there, get your levels up so all your membranes function properly (cell, mitochondria and nucleus – all membranes that need Omega 3’s) and your receptors for Vitamin D can do their job. Oh, and by the way, get it measured and don’t settle for less than great in this most important level.”

This will go a long way to help you end the wanting and needing of better health, lower body fat, better moods, less joint pain and clearer thinking.

The world’s best fish oil lives here.

And the world’s best test for the all-important Omega6/3 ratio’s lives out there on the big old internet.

Get on the fish oil for 6 weeks, then do the test and increase your dose if you need to, to get yourself into the “excellent” level.

Your future depends on it!

Doc

 

Speaking with Giants

I had the very great pleasure of interviewing Dr Elissa Epel a few evenings ago.  As you may recall she is the co-author of the book “The Telomere Effect” along with Elizabeth Blackburn. Blackburn was awarded the Nobel Prize in 2009 for her work on telomeres and telomerase.

My conversation with Dr Epel will be available to you shortly but I wanted to give you a brief low down on some of what we covered. As a clinical psychologist with an interest in stress and its effects on behavior and health, there is a wealth of great advice on how to deal with it, both in the book and on the telecon recording.  Naturally there is nothing better than getting if from the mouth of the author but of course it’s not possible to take several hundred pages and condense them into a one hour interview.

One amusing analogy came out in the discussion.  We spoke about rumination and ruminants. You may know that cows are ruminants because the chew cud, or regurgitate stuff up from their bellies and chew it again and again for digestive purposes.

Well it occurred to me that humans are “cognitive ruminants” because we chew and re chew on our thoughts. Something like 95+ percent of our thoughts are “rethought” over and over again.  If they are happy and joyful thoughts this may not be a problem, but if they are problem or task oriented it does us no good and perhaps harm. Frankly I noticed this patter in my own thinking in the past and have tried to effectively deal with it.  Rethinking a process or answer to a problem rarely adds anything but anxiety once you’ve come up with a plan.

Dr. Epel reviewed some great meditation tips as well to break the cycle of the daily “fight or flight” response and get you out of the limbic mind.

Speaking of giants and minds, my friend Matt Furey recently made a comment about Tai Chi, stating that one of the main goals of Tai Chi was to help you relax! Now that has movement associated with it but he followed it up with a comment about releasing people from the limbic mind.

It was so very fascinating to me that 2 people from 2 dissimilar backgrounds both of whom are among the most self-actualized people I know, came up with the same observations and statements about the limbic mind- often referred to as the “lizard brain” because it does not involve thought, only reflexive action. This is also one of the key brain areas for panic, fight or flight and other actions that while really useful when needed, are not where we are meant to spend hours a day.

Now in terms of longevity and health there are a lot of reasons why you will want to control your limbic mind and stop thought ruminations. One the direct damaging effects of these mental processes is on your telomeres, the biologic time clocks of your cells.

Essential point, stay tuned for when we are going to air this interview!

I also want to remind you that TA-65 now has published studies showing a positive effect on your telomeres and that we are again selling* that product. I have been on it nonstop since 2009!

I also want to remind you do EVERYTHING you can to keep those telomeres healthy- this about lifestyle not just pills potions and lotions.  If you have been paying attention over the past 8 years, you have seen the numerous emails and blogs I’ve written about diet, exercise, meditation, etc. and their potential effects on your longevity and telomere health!

Things are revving up here and we’ll soon have more products for you that I think will help but we should all be grateful for the Giants in our lives who teach us, shape us and spoon feed us their knowledge. Now stop rumination and start doing!

Best,

Dr. Dave

*Neither Dr Epel nor any of my guest super stars in ANY Field endorse me, my products, what I sell or what I say and do.  They appear out of kindness and mutually shared scientific interest and to share their Knowledge with YOU!!!

TA-65 Resources

You are presumably here to find out more information on TA-65 telomeres and telomerase. This page contains just a small amount of what I have written about the topics but, many times the questions I get are the same.  Here you will find the answers to many of the more frequently asked questions that I could not cover on the TA-65 page, and answers to the very personal question you should be asking yourself: Does TA-65 really work?

Please note: I kept many of them in “post format” as they were originally published on the internet.

Lengthened telomeres restore immune system to a younger state

New study is the first ever Peer-Reviewed Report of a Telomerase Activator taken by live humans.

New York, NY (September 8, 2010) — A pioneering study published in the Rejuvenation Research Journal today shows that TA-65, a natural Telomerase Activator made and marketed by Telomerase Activation Sciences, Inc. (“T.A. Sciences”), reduces the percentage of short telomeres in immune cells and restores and remodels the aging human immune system to be more like that of a younger individual.

The year-long study of the first 100 clients of T.A. Sciences found that TA-65, a nutritional supplement marketed only through specialized doctors, had been successful in lengthening shortened telomeres.  Telomeres are sequences of DNA, located at the ends of all chromosomes, which serve as cellular clocks of aging. Every time a cell divides, telomeres shorten until they become critically short, and the cell either stops functioning properly or dies.  By activating a gene that is normally turned off, TA-65 has been shown to activate the enzyme telomerase. Telomerase has the unique ability to restore telomere length and is so important that its discovery won the Nobel Prize for Medicine in 2009.

In addition to TA-65’s telomerase activating properties, the study observed a statistically significant decline in senescent cytotoxic (CD8+/CD28-) T cells. This resulted in the human subjects’ immune systems being restored to a more youthful state. A rejuvenated immune system holds great potential to help subjects whose immune systems have been weakened by a lifetime of various stresses and age related decline.

“Telomere  science  is  relatively  new  and  scientists  are  now  beginning  to  understand  the pervasive role telomeres play in the aging process and how much harm short telomeres cause.” said Noel Thomas Patton, Founder of T.A. Sciences.  “Many studies have established that short telomeres are associated with the decline of the immune system and other major organ systems. Such effects of age-related decline are severe and the benefits of telomere rejuvenation can be profound.  Rejuvenating  shortened  telomeres  will  change  the  nature  of  what  healthy  aging means.”

In  addition  to  taking  TA-65,  which  is  a  natural  product  derived  from  the  Chinese  herb Astragalus, subjects were given a comprehensive dietary supplement pack, and physician counseling.

The study was led by Calvin Harley, Ph.D, and co-authored by Dr. Weimin Liu, Dr. Maria Blasco,  Dr.  Elsa  Vera,  Dr.  William  Andrews,  Dr.  Laura  Briggs,  and  Dr.  Joseph  Raffaele. Several hundred clients of T.A. Sciences have now received TA-65 without any reports of serious adverse events.

Rejuvenation Research

A Natural Product Telomerase Activator As Part of a Health Maintenance

Program

To cite this article:

Calvin B. Harley, Weimin Liu, Maria Blasco, Elsa Vera, William H. Andrews, Laura A. Briggs, Joseph M. Raffaele. Rejuvenation Research. -Not available-, ahead of print. doi:10.1089/rej.2010.1085.

Calvin B. Harley,1,6

Weimin Liu,2

Maria Blasco,3

Elsa Vera,3

William H. Andrews,4

Laura A. Briggs,4 and

Joseph M. Raffaele5

1Geron Corporation, Menlo Park, California.

2TA Sciences, New York, New York.

3Spanish National Cancer Center, Madrid, Spain.

4Sierra Sciences, Reno, Nevada.

5PhysioAge Systems, New York, New York.

6Present address: Telome Health Inc., Murphys, California.

Address correspondence to:

Calvin B. Harley

Telome Health, Inc.

1177 Sandalwood Dr. Murphys, CA, 95247

Abstract

Most human cells lack sufficient telomerase to maintain telomeres, hence these genetic elements shorten with time and stress, contributing to aging and disease. In January, 2007, a commercial health maintenance program, PattonProtocol-1, was launched that included a natural product-derived

telomerase activator (TA-65®, 10–50 mg daily), a comprehensive dietary supplement pack, and physician counseling/laboratory tests at baseline and every 3–6 months thereafter. We report here analysis of the

first year of data focusing on the immune system. Low nanomolar levels of TA-65® moderately activated telomerase in human keratinocytes, fibroblasts, and immune cells in culture; similar plasma levels of TA-

65® were achieved in pilot human pharmacokinetic studies with single 10- to 50-mg doses. The most striking in vivo effects were declines in the percent senescent cytotoxic (CD8+/CD28−) T cells (1.5, 4.4,

8.6, and 7.5% at 3, 6, 9, and 12 months, respectively; p = not significant [N.S.], 0.018, 0.0024, 0.0062)

and natural killer cells at 6 and 12 months (p = 0.028 and 0.00013, respectively). Most of these decreases were seen in cytomegalovirus (CMV) seropositive subjects. In a subset of subjects, the distribution of telomere lengths in leukocytes at baseline and 12 months was measured. Although mean telomere length did not increase, there was a significant reduction in the percent short (<4 kbp) telomeres (p = 0.037). No adverse events were attributed to PattonProtocol-1. We conclude that the protocol lengthens critically

short telomeres and remodels the relative proportions of circulating leukocytes of CMV+ subjects toward the more “youthful” profile of CMV− subjects. Controlled randomized trials are planned to assess TA-65®- specific effects in humans.

PEOPLE TAKE DIETARY SUPPLEMENTS with the intent to preserve mental, physical, and emotional health and vigor into old age. Although drugs and surgical procedures that target diseases of the elderly will hopefully arrest or partially reverse tissue damage caused by aging and chronic stress, measures to maintain health are arguably a better approach to lengthening our healthy life span. Most dietary supplement programs include combinations of vitamins, antioxidants, and other constituents, some of which have been shown to have significant health benefits in controlled clinical studies, whereas others may show adverse effects,1–6 underscoring the need to assess functional effects of combination products. This paper presents initial data from an ongoing observational study of a novel dietary supplement program, PattonProtocol-1, which

includes a natural product-derived telomerase activator targeting a fundamental aspect of cellular aging.

Telomerase is an enzyme that synthesizes the specific DNA sequence at telomeres, i.e., the terminal DNA at the ends of all chromosomes.7,8 Telomeres are essential genetic elements responsible for protecting chromosome ends from being recognized as “broken DNA.” Because telomeric DNA cannot be fully replicated by conventional DNA polymerases, and because telomeres undergo degradative processing and are a “hotspot” for oxidative damage,9 telomeres will gradually shorten with time and cell division unless there is sufficient telomerase activity to maintain telomere length.

Telomerase is activated in fetal development, thus protecting telomeres from significant loss during this period of dramatic cell expansion.10,11 However, telomerase is repressed before birth in most somatic tissue, and, as a consequence, birth marks the beginning of telomere erosion in most tissues throughout life. Tissues with continual cell turnover or periods of rapid proliferation are “telomerase competent” in that they upregulate telomerase during early phases of progenitor expansion.12,13 All adult somatic stem cells appear to be capable of activating telomerase during tissue regeneration. However, these periods of activation are insufficient to prevent telomere

loss, and this is compounded by a decreased ability to activate telomerase during aging and stress.14–16 In addition, stress can accelerate telomere loss by increasing cell turnover and the amount of telomeric DNA lost per cell division.17,18

In cross-sectional studies, humans lose telomeric DNA at a very modest rate of about 15–60 bp per year, likely reflecting the small numbers of stem cells that are actively dividing in proliferative tissues compared to the total stem cell reserve, and the quiescent state of cells in other tissues. Telomere shortening has been investigated in human cells in culture, in human genetic diseases with mutated telomerase, and in animal models of telomerase deficiency.13,1926

These studies point to a causal relationship between telomere loss, cell aging, reduced tissue regeneration, and loss of tissue structure and function. In support of this causal relationship, epidemiological studies show that short telomeres in humans are a risk factor for atherosclerosis, hypertension, cardiovascular disease, Alzheimer disease, infections, diabetes, fibrosis, metabolic syndrome, cancer, and overall mortality.18,24,25,2730

Chronic viral infections such as cytomegalovirus (CMV) and human immunodeficiency virus (HIV) accelerate telomere loss and premature aging of the immune system, especially the virus- specific cytotoxic T cells31–36 responsible for killing infected cells. In addition to telomere loss, these cells often lack expression of the co-stimulatory receptor CD28 and have reduced proliferative capacity, reduced ability to secrete antiviral cytokines and chemokines, increased

resistance to apoptosis, and compromised ability to lyse infected cells. About 50% of the U.S. population is infected with CMV as judged by circulating CMV-specific antibodies, but after an initial 30% seropositivity rate by age ≈10, there is ≈1% annual seroconversion rate throughout life leading to ≈90% seropositivity by the ninth decade. This linear increase has made it difficult to distinguish the effects of pure immunosenescence from those that can be attributed to this extremely common virus.37,38

Here we report initial findings from a dietary supplement program which includes TA-65®, a purified small- molecule telomerase activator derived from an extract of a plant commonly used in traditional Chinese medicine. Telomerase activation and functional studies on a related molecule (TAT2) from the same plant have been previously reported for human skin keratinocytes and immune cells in culture.36 Effects of

TAT2 in tissue culture studies with CD8+ T cells from HIV/acquired immunodeficiency syndrome (AIDS)

subjects included increased replicative capacity, improved cytokine and chemokine responses to antigens, and increased killing of autologous HIV-infected CD4+ cells.

PattonProtocol-1

PattonProtocol-1 was launched in January, 2007, by TA Sciences (New York) as a commercial age-management product composed of a natural product–derived telomerase activator (TA-65®, described below), a dietary supplement pack (online material S1), laboratory testing (Table 1), and physician counseling. All subjects signed a comprehensive Customer Acknowledgement

Form. Baseline assays (Tables 1 and 2) indicated that most individuals were within the normal ranges for the majority of tests. In a small number of cases described in the  Results section, the consulting physician prescribed medications for subjects based upon clinical tests. There was no qualitative change in the overall conclusions whether these subjects were included or censored from the analysis. We report results for all evaluable subjects who completed 12 months of the protocol by June, 2009. The number of subjects at 3, 6, 9, and 12 months for most tests was 43,

59, 27, and 37, respectively. The age and gender frequencies of the subset at each time point were similar to those of the total baseline population (n = 114; 63 ± 12 years, 72% male).

TA65®

TA-65®, exclusively licensed to TA Sciences from Geron Corporation, is a >95% pure single chemical entity isolated from a proprietary extract of the dried root of Astragalus membranaceus and formulated into 5- to 10-mg capsules with inert excipients. Starting doses of 5–10 mg/day were considered safe on the basis of historical usage of extracts. Some subjects increased their dosage after several months on the product to 25–50 mg/day. Cumulative dose consumed during the year was recorded for each subject and used for preliminary dose–response analysis.

Clinical laboratory assays

At baseline and each time, point blood samples were drawn and shipped the same day at ambient temperature to analytical laboratories. Assays for standard blood counts, blood chemistry, specialized immune subsets, CMV antibody titer, and inflammation markers were conducted at Quest Diagnostics or Bio-Reference Laboratory. Specialized immune subset analyses were conducted at UCLA Clinical Laboratories and Pathology Services.

Telomerase activity assay in cultured human keratinocytes and fibroblasts

Telomerase activity was measured in human neonatal keratinocytes (Cascade Biologics, Portland, OR) and in MRC5 fetal human fibroblasts (ATCC# CCL-171) pre-and posttreatment in culture with TA-65® using the telomere repeat amplification protocol (TRAP) assay, essentially as described elsewhere.39 In brief, telomerase activity was measured in actively growing cells incubated for 24–48 h with TA-65® in the vehicle (dimethylsulfoxide [DMSO] at 1% vol/vol [keratinocyte culture] or 0.5% [fibroblasts]) versus vehicle alone. Measurements were typically made at 5–10 population doublings (PD) (keratinocytes) or 30–40 PD (fibroblasts). Telomerase reaction products were resolved by electrophoresis on nondenaturing polyacrylamide gels and quantified by exposure to Phosphor Screens and imaging on PhosphoImager SL

(Molecular Dynamics).

Telomere length assays

Median telomere length in peripheral lymphocytes and granulocytes was determined by FlowFISH at Repeat Diagnostics (Vancouver, Canada) essentially as described elsewhere.40 Mean telomere length by qPCR41 was performed in the laboratory of Dr. Richard Cawthon (University of Utah, Salt Lake City, UT). High-throughput quantitative fluorescence in situ hybridization (qFISH)42 was performed at CNIO, Madrid,

for inter- and intranuclear telomere length distributions.

Statistics

Data from this study were collected primarily as a hypothesis-generating exercise because subjects were not participating in a controlled prospective study, and statistical analyses were not formally defined a priori. Baseline data were analyzed for cross-sectional age effects. Student t-tests were used for comparison of means and the F-distribution for significance of linear regression against subject age. Except where indicated, two-tailed paired t-tests were conducted at each time point for comparisons to the baseline values. For percentage of short telomeres analyzed by HT qFISH, individual differences between baseline and postproduct data were analyzed by chi-squared analysis.

Mechanism of action

TA-65® activates telomerase in human neonatal keratinocytes and fetal fibroblasts in culture

TA-65® upregulates telomerase activity in low- and mid-passage human neonatal keratinocytes two- to three-fold in a dose-responsive manner (Fig. 1A). In these studies, activation was two- to three-fold at the lowest concentrations tested (30–100 nM), and activation was not as great at higher concentrations. This pattern is similar to that seen for TAT2, a related molecule tested in human immune cells.36 The ability of TA-65® to upregulate telomerase activity was also tested in

human fetal fibroblasts (MRC5) over a broad concentration range (Fig. 1B). Untreated and vehicle (DMSO)-treated MRC5 cultures showed extremely weak telomerase activity; there was essentially no telomerase extension products with a size greater than that of T1, the minimum size needed to detect a product in the TRAP assay. Results from three independent experiments

indicated that TA-65® at concentrations as low as 1 nM induced processive telomerase activity in MRC5 cells. Telomerase activation by TA-65® in the 1–30 nM range in multiple cell types is important, because plasma levels of TA-65® are typically in the 1–20 nM range 4–8 h postoral ingestion of 5–100 mg TA-65® (unpublished data).

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FIG. 1.     Telomerase activation by TA-65® in neonatal foreskin keratinocytes and fetal lung MRC-5 fibroblasts. (A) Keratinocytes in triplicate wells were exposed for 48–72 h in different experiments to the dimethylsulfoxide (DMSO) vehicle control, epidermal

growth factor (EGF) (positive control, typically 10 ng/mL), or TA-65® at indicated concentrations, and products were analyzed as described in  Methods. Results from analysis of telomere repeat amplification protocol (TRAP) ladders resolved by gel electrophoresis and quantified by ImageQuant on a PhosphoImager are shown for a

typical experiment. (B) MRC-5 cells were exposed to TA-65® at concentrations shown

for 48 h. Each replicate represents an independent lysate (a replicate culture dish within one experiment). “Chaps” represents the lysis buffer control (no cell extract). HeLa cells are used as positive control cells as described in  Methods. T1 is the first telomerase extension product capable of amplification by PCR. IC is the internal control PCR product. Shown is a representative gel from three independent experiments.

Baseline observations

The relationships between age and various biomarkers including telomere length have been reported in a number of cross-sectional studies.  Table 2 shows mean values, standard deviations, count, slope, and R2 from linear regression on subject age, and the statistical significance of the slope for the baseline tests investigated in this report. As expected, this population showed a

highly significant decline as a function of client age in both lymphocyte and granulocyte

telomere length by FlowFISH analysis, and the slopes of the decline (55 and 34 bp/year; p = 10−15

and 10−8, respectively) are comparable to those reported previously.43–45 Age-dependent increases are seen in the percent senescent (CD8+CD28−) cytotoxic T cells, percent natural killer (NK) cells, and percent and absolute number of neutrophils. Significant age-dependent decreases are seen in naïve (CD8+CD95−) cytotoxic T cells, B cells, and lymphocytes.

Because CMV infection can have a significant impact on immune markers,37,46 we analyzed the age-dependency of baseline immune subsets by CMV status (Fig. 2 and  Table 3). Lymphocyte, but not granulocyte, telomere length was longer in CMV− subjects than that in CMV+ subjects, suggesting that CMV infection drives increased turnover (and hence telomere shortening) in lymphocytes, but has relatively little effect on hematopoietic stem cells. Telomere length in granulocytes is considered a surrogate of telomere length in hematopoietic stem cells due to their short transit time to peripheral blood, and short half-life in circulation.42 The ages of the CMV+ and CMV− subjects (65 ± 12 and 62 ± 13, respectively) were not significantly different, but the mean lymphocyte TL in CMV+ individuals was 680 bp less than that observed in the CMV−

group (p = 0.003), suggesting an acceleration of aging by about 10 years in the CMV+ group

based on −55bp per year for lymphocytes.

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FIG. 2.     Baseline telomere length and immune subset data as a function of subject age segregated by cytomegalovirus (CMV) status. Overall baseline data without segregation by CMV status is provided in  Table 2. Correlations with age for lymphocyte and granulocute telomere length (A,B) and immune subsets (C–J) at baseline were

determined by linear regression in the CMV+ and CMV− subjects. Telomere length and immune parameters were analyzed by flow cytometry as described in  Methods.

As expected, baseline numbers and the rate of increase in senescent cytotoxic T cells (CD8+/CD28−) in percent and absolute counts was highest in the CMV+ population (Table 3 and Fig. 2C,D). The slight increase in per cent of senescent cytotoxic T cells as a function of age in CMV− subjects (Fig. 2C) despite declining absolute numbers of these cells (Fig. 2D) is a consequence of a significant decline in total CD8+ cells as a function of donor age in CMV− subjects (Fig. 2 E,F). The difference in the mean number of CD8+ T cells between CMV+ and CMV− subjects (208 cells/μL) is essentially accounted for by the difference in mean number of senescent CD8+ T cells between these two subpopulations (200 cells/μL) (Table 3). Although the

%CD8+CD28+ cells at baseline was significantly higher in CMV− subjects (p < 0.0001), this was due primarily to the elevated absolute number of CD8+CD28− cells in the CMV+ subset (i.e., an increased denominator for the CMV+ group). The absolute number of nonsenescent cytotoxic T cells (CD8+CD28+) was not significantly different between CMV+ and CMV− subjects (Table 3).

CMV+ subjects had significantly fewer absolute and percent naïve cytotoxic T cells (CD8+CD95−) at baseline than did CMV− subjects (p < 0.0005 and 0.07, respectively) (Table 3), but in both populations there was dramatic reduction in the absolute and relative abundance of

these cells as a function of subject age (Fig. 2 G,H), consistent with previous studies.47,48 In 80- to 90-year-old subjects, there were <50 naïve CD8+ cells/μL.

A novel finding in the baseline dataset is an apparent effect of CMV infection on neutrophils: CMV− subjects show an increase in neutrophil number and percentage as a function of subject age compared to an essentially flat profile with age for the CMV+ subjects (Fig. 2I,J). At baseline, the mean number of neutrophils in CMV− subjects was about 20% higher than that in CMV+ subjects (p = 0.02 by absolute counts, p = 0.003 by %) (Table 3). There was also highly significant increase in baseline percent (p =

0.00015) and absolute numbers of NK cells (p = 0.006) in the total population (Table 2), but there was no significant difference between CMV− and CMV+ subjects.

Changes from baseline

Reduction in percent cells with short telomeres

Two independent measures of median or mean telomere length (by FlowFISH and qPCR) showed no consistent change with time on PattonProtocol-1 (data not shown). However, we also analyzed the distribution of individual telomere lengths using automated high-throughput confocal microscopy (HT qFISH42). Telomere signals within the nuclei of white blood cells were analyzed from 13 subjects at baseline and a follow-up time point between 12 or 18 months.

Mean telomere length by HT qFISH correlates relatively well with median telomere length by FlowFISH (supplemental data S2) and also showed no consistent change of overall telomere length (Fig. 3A). However, HT qFISH revealed a decline in the percentage of nuclei with short telomeres (<4kbp) in 10 of the 13 individuals (p < 0.05 for 7 of those 10) at 12–18 months compared to baseline, while only one of the remaining 3 individuals had a significant increase in percent short telomeres (Fig. 3B). Given these data, we used a one-tailed paired t-test to determine the probability that the overall mean reduction across all 13 subjects was due to

chance (p = 0.038). In separate studies in murine cells in culture and in vivo we have shown that TA-65® alone will reduce the percentage of cells with short telomeres with minimal effects on mean telomere length (M.B., manuscript in preparation).

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FIG. 3.     Mean telomere length and percent of nuclei with short telomeres at baseline and post TAS protocol. (A) Mean telomere length values ± standard error (SE) of the indicated individuals at base line (black bars) and post PattonProtocol-1 (grey bars)

as determined by high-throughput quantitative fluorescent in situ hybridization (HT

qFISH). The total number of nuclei analyzed is indicated (n) on top of each bar. Statistical significance was assessed by the Student t-test. (B) Percentage of nuclei with short (<4 kb) telomeres at base line (black bars) and post-TAS protocol (grey bars) as determined by HT qFISH. Numbers above bars represents the number of nuclei with short telomeres (<4 kb) out of the total number of nuclei analyzed. The chi- squared test was used to evaluate the statistical significance for each individual tested.

Positive remodeling of the immune system

There were a number of striking changes from baseline in the adaptive and innate immune system of subjects on PattonProtocol-1. We saw statistically significant “age-reversal” effects in the number and percent of senescent (CD8+CD28−) cytotoxic T cells, particularly after 3 months

(Fig. 4A). Senescent CD8+CD28− T cells dropped with a linear trend from roughly 39% in the baseline population to about 36% at 12 months in the overall population (p = 0.0068 at 12M). Most of this effect was due to a 20% drop in the number of CD8+CD28− cells in the CMV+ population (Fig. 4D, p = 0.0044 at 12M). This decrease, relative to the age-related increase of

0.57%/year and 3 CD28− cells/year in CMV+ individuals, represent an apparent age reversal of

≈5–20 years in this biomarker of immune aging.

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FIG. 4.     Relative changes from baseline as a function of time (months) for immune subsets. The mean of the absolute change from baseline for each parameter across all subjects for which data at that time point was calculated and then expressed as a percent change from the mean value at baseline for those

subjects. In A–C, data for both cytomegalovirus-positive (CMV+) and CMV−

subjects are combined. (D) Relative mean change is shown for the CMV+

population subpopulation only. Asterisks next to a data point signifies p < 0.01 (***), p < 0.05 (**), and p < 0.1 (*) for a two-tailed paired (within-subject) t-test analysis comparing baseline to time point values.

At baseline, CMV+ individuals had a significantly lower number and percent of neutrophils compared to CMV− individuals (Table 3). After 3 months on PattonProtocol-1, there was an overall increase in number and percent of neutrophils (Fig. 4B) which was primarily driven by the effects of the Protocol in the CMV+ subjects (Fig. 4D), suggesting that as in the case with CD28− cells, PattonProtocol-1 may reverse a potentially detrimental effect of CMV infection on neutrophils.

The percent and number of NK cells significantly decreased after about 6 months on product (Fig. 4C). A decrease was observed in both CMV+ and CMV− individuals, but again the effect was more pronounced in the CMV+ population (data not shown). The changes at 12 months in the overall population (about a 15% decline in both % and number of NK cells) was highly significant (p = 0.0035 and 0.0021 for number and per cent cells, respectively), and dramatic compared to the baseline increases per year in NK cells for the

CMV+ group (+2.4 cells per year and +0.3% per year).

The inability to maintain telomeres with age and chronic stress has been linked to declining health and the increased risk of disease and death from many causes, including cancer.16,25,49–52

In this study, we report that a 1-year health maintenance program consisting of a dietary supplement pack combined with a natural product–derived telomerase activator results in a decreased percentage of short leukocyte telomeres and remodeling of the relative proportions of the circulating leukocytes of CMV+ subjects toward the more “youthful” profile of CMV− subjects.

One of the strengths of our study is the low CMV-positivity rate (54%) in a relatively older population, which allows us to separate the effects of age and CMV status on immunosenescence. It also serves to mitigate one of our study’s weaknesses—the lack of a control group—as the subjects were initially unaware of their CMV status and their subsequent knowledge is unlikely to have caused the segregation of many of the effects of the protocol by CMV status.

Our description of the decrease in CD8+CD28− T cells as a positive remodeling of the immune system is supported by the increased morbidity and mortality associated with what is known as the immune risk profile (IRP). This profile has been defined as a CD4/CD8 less than 1 in association with CMV seropositivity by longitudinal studies of individuals in their eighties and nineties in the OCTO/NONA Swedish cohort.53 As in our cohort, the major driving force for the decreased CD4/CD8 in CMV+ subjects in this population is likely the accumulation of virus- specific CD8+CD28− T cells. These studies have reported 6-year follow-up data, and no individuals who have survived to 100 years old exhibit the IRP, even if they are CMV+. The authors conclude that successful immune aging entails being able to control CMV infection without accumulating senescent cytotoxic T cells. Thus, we conclude that the 20% reduction in CD8+CD28− T cells is a salutary effect, even though we have yet to see increases in the number of CD8+CD28+ T cells. Telomere shortening associated with replicative senescence is the probable cause of loss of CD28 expression and apoptosis resistance of CD8+ T cells.54 The

decrease in the percentage of short telomeres we found makes upregulation of telomerase by TA-

65 the most likely mechanism for this salutary effect.

Age-related changes in the innate immune system have not been as well characterized as those of the adaptive immune system, although the importance of changes in the former is increasingly being recognized.55 It is generally agreed that the per-cell activity of neutrophils as measured by oxidative burst, phagocytosis, and chemotaxis decreases with age.56 There is less agreement on the effect of aging on neutrophil number which has been variously reported to be preserved,57 decreased,58 or increased53 with age. CMV status is not reported in the first two studies, but in

the last study from the above-mentioned NONA cohort, the increase in neutrophil number is based on a comparison between 18 middle-aged (55-year-old) subjects with a 55% CMV+ prevalence rate and 120 very old subjects (92–100-year-old) with a 87% CMV+prevalence rate. Most of the cross-sectional increase of 960 neutrophils occurs within the 92 to 100 year olds, with only a 52-cell increase between 55 and 92. There is also a significant longitudinal increase over a 6-year interval in the very old group. This suggests that there is selection for those very old subjects able to increase their circulating neutrophils in the face of deteriorating tissues, increased inflammation, and increased exposure to infectious agents. Our novel finding that by age 62 the neutrophil number is 20% higher and continues to increase with age only in CMV− subjects can be interpreted as a compensatory increase in the face of declining per cell activity and barrier function, as well as increased antigenic load. The absence of a cross-sectional increase with age in neutrophil number in CMV+ subjects suggests that this compensation is blocked in the CMV+ subjects perhaps due to inhibitory cytokine production by the senescent T

cells. The effect of the protocol to increase neutrophil count in CMV+ subjects can be interpreted as a salutary removal of this block in part through reduction in the number of senescent T cells.

There is a broad consensus that NK cell number increases with age to compensate for decreased per-cell activity, which results from impaired signal transduction,59 but other mechanisms such as decreased barrier function and increased antigenic/pathogenic load may also contribute to increased NK cells with age.60 The decrease in NK cell number induced by the protocol is an “age reversal,” but because we did not measure NK activity we cannot say whether it is from improved barrier function or improved signal transduction. Unlike other cells of the innate immune system, NK cells proliferate after activation and experience further telomere shortening once they are released from the bone marrow.61 The decrease in the percentage of short

telomeres we found could result in improved signal transduction as a mechanism for the reduction in NK cell number. Taken together, these three changes in leukocyte number induced by the protocol represent a remodeling of the immune systems of CMV+ subjects to look more like those of CMV− subjects and successfully aging CMV+ centenarians.

Physicians who monitored the health of the current study subjects through 1 year on the product reported no adverse events that were likely related to the protocol. However, 2 subjects who recently escalated their daily dose reported feeling “anxious” on 100 mg/day but not when they switched back to 50 mg/day. A placebo-controlled study will be needed to determine if this potential adverse effect is real. No new cases of cancer or cardiovascular disease were reported during the overall 260 person-years of dosing with PattonProtocol-1 through June, 2010, and this is statistically significant (p < 0.05, cancer; p < 0.02, CVD) assuming baseline age-specific risks

in our population were similar to those of the U.S. population.

TA-65® activated telomerase in cultured human cells at concentrations seen in the plasma of subjects on the protocol. Paradoxically, although ≈40% of subjects showed an increase in mean telomere length over time, on average across all subjects there was a nonsignificant decline in mean telomere length.

However, we speculate this effect is explained by cell dynamics and the fact that telomerase preferentially lengths the shortest telomeres.62–64 Rescue and selective expansion of near-senescent cells with short telomeres could lead to a reduction in the population mean telomere length despite some lengthening of telomeres in all cells. Because detrimental effects of telomere loss are primarily driven by short,

dysfunctional telomeres, and loss of tissue function and disease onset in proliferative tissues have been associated with telomere lengths <4 kbp,25,65 we believe that our observed reduction in telomeres <4 kbp in subjects on PattonProtocol-1 is a significant, positive response, and that TA-65® contributes to the apparent benefit of the dietary supplement. In support of this, studies with TA-65® given orally in old mice

showed similar reductions in percent cells with short telomeres and positive functional effects on tissues (Blasco et al., submitted) and preliminary dose–response analyses showed an increase in salutary effects with TA-65® doses up to 20–30 mg per day average compared to the initial 5- to 10-mg per day dose

(data not shown). Finally, analysis of additional biomarkers of aging in subjects on PattonProtocol-1 suggest improvements in the cardiovascular system, metabolism, and bone mineral density, which will be

further studied and reported elsewhere. Independent randomized controlled studies with TA-65® alone

are planned.

We thank Drs. Tom Okarma, Spencer Brown, and Rita Effros for critical review of the manuscript, and Noel Patton for patience, encouragement, and financial support of the studies. We also thank the early telomerase activation team at Geron Corporation for generating data in human keratinocytes, Marissa Chunisingh for acting as a key interface between clients and TA Sciences staff, and Drs. Nathan Wong and Sherman Xi for critical feedback and help with statistical analyses.

Calvin Harley is one of the inventors of TA-65. He consults for TA Sciences and is personally taking TA-

  1. 65. He owns stock and stock options in Geron Corporation, a company that is developing telomerase activators for therapeutic purposes and the company that licensed TA-65 to TA Sciences. He is co- founder, President, and CEO, and holds stock in Telome Health, Inc., a diagnostics company that will

provide telomere- and telomerase-related assay services to the healthcare industry. Joseph Raffaele consults for TA Sciences; he is CEO of PhysioAge Systems, LLC, a company that provides biomarkers of

aging analysis, including telomere lengths, to medical practices, and he is co-founder of PhysioAge

Medical Group which offers TA-65 through the Patton Protocol. William H. Andrews owns stock or membership options in Geron Corporation and Sierra Sciences; he consults for TA Science, is a client of TA Sciences who is taking TA-65, and is one of the subjects studied to generate data for this manuscript. He is the founder, President, and CEO of Sierra Sciences, a company that develops therapeutics for inducing telomerase expression and has provided financial support for some of the studies described in this manuscript. Weimin Liu is an employee of TA Sciences. Elsa Vera and Maria Blasco have no competing financial interests.

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1)   The cancer question the post below addresses the reasons for the misconception that TA-65 might cause cancer and the converse: how it might actually benefit or reduce the likelihood of cancer

Does Telomere Science Reveal a Cure for Cancer?

This post is going to be a little more scientific and detailed than I normally send, I hope that is ok, let me

know what you think about this style of education and if you like it I’ll do more.

When we see people who are exceptionally fit or in incredibly good health in their later years we often blame it on having good genes.

And, that is partially true but the part that is true is the part of the genetic material we all share, it is the end of the chromosome called telomerase. Telomeres control how long and how well we live.

Let me explain.

The discovery of telomerase is a real Christmas miracle. It was on Christmas day that Greider and Blackburn finally proved their theory that an enzyme added telomeric DNA to the ends of chromosomes. That was in 1984, and that enzyme was later named telomerase.

Telomerase is present and active in stem cells, germ cells and cancer cells which is one reason these cells never reach senescence (an endpoint where cells die or no longer divide and thus become inert). Normal cells don’t express telomerase. That gene is suppressed in them. One key to immortality is turning that gene on. Thanks to extensive scientific research, a natural activator TA-65 has been discovered and proven to successfully lengthen telomeres.

But every new discovery is initially feared, then embraced by pioneering spirits, and finally adopted by the hungry masses (or in the case of naturally occurring cures, co-opted by corporations to be patented and turned into expensive controlled medicine since they can’t patent nature).

When it comes to telomeres, many fear turning this gene on in healthy cells is unsafe and risky. Their concern stems from what incorrectly appears to be a similarity or link with cancer.

So, what about cancer?  If telomerase is turned on in cancer cells and is the reason a cancer cell continuously replicates, would turning it on in normal cells increase the risk of these cells mutating and becoming cancerous? Specifically is there any evidence that TA-65 causes cancer?

The answer is no and I’ll tell you why.

Stimulating growth of telomeres on normal chromosomes does not cause them to become cancerous because cancer cells are by definition abnormal cells. This bears repeating. It DOES NOT cause normal cells to become cancer cells.

As I explained, cancer cells are abnormal cells. When your genetic material (DNA) becomes damaged or is altered, it mutates and affects normal cell growth and division.  Activating telomerase to lengthen telomeres does not alter or damage our DNA. Scientists have turned on the telomerase of normal cells ATTEMPTING to turn these cells into cancer cells and have been repeatedly unsuccessful.

For the past few decades telomerase research was almost solely focused on creating a “telomerase inhibitor” that could be a potential cure for cancer. Because 80% of all human cancers are immortalized by the turning on of telomerase it’s a logical conclusion that preventing the immortality of cancer cells would slow growth and allow chemotherapies to work more effectively.

I support this research, but when TA-65 was discovered a decade ago and has been shown to be safe and effective as a telomerase activator in healthy cells then the research focus and possibilities expanded.  It’s with these new possibilities that I am most intrigued by and signal health and vitality for all long into our “golden years”.

We know that when cells approach senescence (their endpoint), the short telomeres stimulate the chromosome instability which can cause the mutations that are responsible for creating cancerous cells. If we lengthen the telomeres and extend the life of the cell, we thereby reduce the risk of chromosome rearrangement (where tumor-suppressor genes can be shut off and cancer-causing genes turned on). Telomerase has also been shown to extend the life span of our immune cells, which improves their ability to seek and destroy cancer cells.

According to the American Cancer Society, 78% of all cancers are diagnosed in people 58 years and older.  Cancers are more likely to occur when signs of cellular senescence are present and when the immune system begins to be compromised with age and lose its ability to respond to threats. It follows then that extending the period of time before normal cells reach senescence and before immune cells die, would help prevent the creation and spread of cancer in our bodies.

It’s important to note that the unbridled and aggressive way telomerase is activated in cancer cells is

also very different from the gentle and mild activation of telomerase in stem-cell and germ-cell lines. TA-

65 mimics the natural and gentle activation of telomerase in stem-cells and germ-cells. In 2002, testing and research began with TA-65 and to date there are no studies linking its use to cancer. Rather studies showing its ability to increase the number of “good guys to fight off the bad guys”, gives hope that it will assist our bodies when fighting off cancerous mutations.

In addition adding TA-65 to human non immortal cancer cell lines, adding TA-65 to pre malignant cells ( cells on their way to becoming cancer), adding to-65 to highly cancer prone mice and of course giving TA-65 to people has shown no increases in cancer. AS far as prevention that has not yet been thoroughly studied but many of us in the longevity field believe it holds promise for reducing the risk of cancer.

This next one is a follow up to the where does it come from and how does it work question

TA-65 is derived from the Astragalus root, a natural compound used and researched for its ability to measurably boost the immune system. Before it was released as a supplement it was studied for effectiveness against various viruses and proved beneficial against HIV to CMV viruses, among others. These viruses are associated with increased cancer rates, and because of this the question is often posed, “could a telomerase activator actually improve cancer outcomes and maybe even be a cancer preventative?”  Geron, the parent company that produces TA-65, is commencing tests and attempting to answer that question.

I believeTA-65 is currently offering cutting edge hope for those pioneering souls who want to explore the boundaries of living fully, healthfully, as long as possible.

A key to remember about living longer is that while the number of years you live is important, the number of years you live fully, with great brain and body function is the real measure of longevity. This is called health span. Most people, scientists, doctors and laymen alike agree, maximizing health span is

more important than maximizing life span. Think of it this way, your life can be extended for quite some time with life support but most of us wouldn’t chose to live for months or years on life support.

With TA-65 and Telomerase activation increasing life and health span is now within the reach of almost everyone. I speak with people every day who are exploring ways of extending their health span. If you have specific questions around TA-65 and whether or not it might be effective for you, visit my website for info on contacting me, I’ll be happy to answer your questions.

And please do let me know in the comments below if you like these more technical, educational pieces,

if so, I’ll get more of them out right away.

Best,

Dr. Dave

WHAT about RESVERATROL and TA-65?

If you have not read “The Immortality Edge” I would suggest you head on over to Amazon and do so as it will give you very succinct recommendations.

As far as resveratrol is concerned I remain of this opinion: it’s a decent antioxidant so you are probably not wasting your money. I think 250 mg is a low dose though. Also as I have publically stated i sent several brands off to be tested independently for telomerase activity and they had no activity on telomerase at all so I do not believe it is effective for that purpose in free living human beings.

Additionally just yesterday the whole mechanism of Resveratrol has been question. It may not work through the sirtuin pathway at all but rather through adipokines which would put it squarely in the “antioxidant” category.

It’s great to see the interest in telomeres telomerase and longevity. I want to make one thing very clear. Very few people (although it’s not ZERO) tell me they want to live forever. But almost everyone tells me they want to be healthy for as long as possible. To me that is what the most immediate promise of telomere biology is. There is also phenomenal progress in telomerase inhibition for those who already have cancer. Just last year parent company Geron did two big things. They released the data on their inhibitor Imelestat, which was very promising. And second, they granted an exclusive international patent to TA Sciences for distribution of TA-65.

But as fate would have it, telomerase inhibition is looking more and more like a dead end and telomerase activation may hold the most promise for cancer prevention. So for those of you who are

still convinced that Resveratrol may have some activity as a “telomerase inhibitor” please read the following very carefully!

In my opinion ALL telomerase inhibitors will ultimately fail as cancer treatments. Why?

1) Because it is now widely understood that telomerase activation, especially the “on-off” type of activation provided by TA-65, does not cause cancer. It has been at least 7 years since any scientific publication has even mentioned the association between telomerase expression and cancer but some people still propagate the myth on the internet.

2) Telomerase inhibition as a therapy has a terrible side effect. Most cancers “figure out” an alternative way to keep their telomeres long. As a matter of fact, that way is actually called “ALT”, short for alternate. When a cancer becomes a cancer, the last step is to turn on telomerase in huge

and continuous amounts so the cancer can survive its own unrestrained growth but there is always the

ALT mechanism lurking in the background.

When you inhibit telomerase, you get rid of a giant chunk of cancer cells. Good, right?!

Not so good; as a matter of fact, deadly. You’ve now given the lurking ALT cells no competition and they will grow in unbounded fashion. Sadly, they are EVEN WORSE than telomerase expressing cells. So you’ve opened the door for ALT cells to grow with no competition. What happens in clinical trials? Those cells take over after telomerase inhibition and the cancer comes raging back and is even more resistant to treatment.

Most of us in the field feel that in order for telomerase inhibition to have a chance you need an

immune booster to come in and clean up the left over mess (those ALT cells) once you’ve gotten rid of the telomerase dependent ones.

Now I cannot say that TA-65 prevents or fights cancer, but I can say it boosts the immune system.

It has shown this power in cells, tissues, mice and finally, humans. The response is amazingly consistent.

This, coupled with the fact that cancer is associated with short telomeres, has led some clinicians, including myself, to use it in cancer patients.

Personally, I would not wait until you have cancer to take it!!! Dr Dave

These statements have not been evaluated by the FDA. TA-65 is not a drug and is not intended to diagnose treat or mitigate any disease process including cancer. Always consult your physician before starting or changing any medicines or supplements.

HOW DO I GET TA-65?

Here is a link to more info on TA-65 TheLongevityEdge.com/

I want to stress that TA-65 is not strong enough to immortalize human cells but it is strong enough to lengthen the short (dangerous) telomeres in rather dramatic fashion. I think this may account for the safety of the molecule which is extraordinary.

WHAT IS TA-65 and is there a cheaper version or something else that works as well?

As to its composition. it is not Astragaloside IV which I tested and it has no activity on telomerase. And it is not cycloastragenol either which some people have claimed. It is known as a “related molecule to” TAT2 in Geron circles but the exact composition is carefully guarded and internationally patented. The molecule itself it found in one specific type of Astragalus root located in one specific

area of Mongolia. It takes approximately 4 tons of Astragalus root to make one typical ( medium dose)

yearly course of TA-65 so I hope this answers some of the questions about substitution and cost.

By the way even though I wrote the TAS manual and spoke at 2 major anti-aging conferences on telomeres and was a major contributor to the only “bookstore” book on the topic “The Immortality Edge” and am one of the few distributors in the country licensed to sell the product I do not work for TA Sciences and I have no say in the cost of the molecule.

As to who was first in the country I am clear the first doctor to be on it and distribute I was Dr Fred Vagnini a 7 time bestselling author and a cardio thoracic surgeon turned diabetes and weight loss expert. Dr Sears is indeed one of the early adaptors of the product and I have had the pleasure of swapping “war stories” with him over lunch and dinner at some of the conferences. As a person I like Al very much.

For those of you who really like this topic poke around these people’s web sites. In the meantime I will

crank out more stuff for you and don’t forget to check my blog. DD aka Dr Telomere

HOWCOME THERE ARE SO MANY SUPPLEMENTS THAT CLAIM TO TURN ON TELOMERASE?

The short answer to why research studies show telomerase activation where none exists is as follows

1) Operator inexperience. As I mention in prior posts I actually sent stuff straight to Sierra Sciences the experts in the field to be tested and I share those results in the book at length and shorter versions in these posts.

2) Using genetically modified cell lines

3) Measuring telomerase surrogates and sub units. The entire complex is required. Often increased mRNA templates are seen in the absence of actual telomerase activity

4) Overly optimistic interpretation of results known as bias.

5) Using cell lines that already express measurable telomerase without any other outside agents needed. The gold standard for telomerase expression is human fibroblast cultures since these are extremely hard to induce. If you get results in these you can be sure you have something real; this is where Ta-65 was tested.

6) Using a telomere test with poor precision or accuracy. This is probably the most common when it comes to telomere length especially in shorter studies.

7) What I call the internet misinformation machine. Example Dr X is super successful and has a highly

paid copy writing team and a giant list of adoring followers who don’t question or research anything that comes from him. That team writes something that is not accurate or is downright false either intentionally or unintentionally. Dr X is busy on a Hawaiian vacation and does not notice the inaccuracy. It gets published generates a good sales response and is distributed via Dr X’s vast network of affiliates to many blogs and posts on the internet. Everyone is looking for interesting and stimulating content for their blogs. The affiliates of the affiliates scrape the blog content and pretty soon you have an article that quotes an article that quotes an article etc etc.

You have just witnessed the birth of a FACTOID. There are few areas where there are more factoids than the area of telomeres and telomerase.

Keep in mind telomerase expression is a good thing but often does not equate to actual telomere elongation. That is why TA-65 stands out. In the most meaningful test of all short telomere testing in live free living humans as well as several objective improvements in things like immune function, bones density, glucose etc etc.

No other telomerase supplement has been tested that way. Also there is a difference between slowing down telomere loss and actually lengthening telomeres. A lot of studies that look at humans and supplements are matched to control groups for comparison with nobody having baseline starting telomere lengths in either group. For example: nonsmokers have longer telomeres than smokers. This gets reported as “Not smoking lengthens your telomeres”.

Compared to what. In order to say you actually ADD length versus slowing down loss you need a baseline telomere length in the same people as you are testing your supplement/intervention in. If they are longer after the supplement or intervention then you have proven you lengthened telomeres.

A classic example is a recent study that showed multi vitamins added 5% length to telomeres in women who took them versus women who did not. The conclusion’s often reported as “multivitamins lengthen your telomeres”. More than one doctor on this very site posted that type of interpretation. Again what that study really showed was that multivites probably slow down telomere loss since there was no baseline in either group to prove that actual length was added.

Notice how different this is from slowing down loss. Now don’t get me wrong slowing down telomere

loss is SUPER important and should be done IN ADDITION to adding length whenever possible.

Also notice that turning on telomerase is not the gold standard, changes in telomere length are. You can turn on telomerase and not add any length at all and possibly not even slow down the loss. The reasons for that are quiet complicated just remember: turning on telomerase does not automatically equal longer telomeres.

The only supplement that has been shown to add length to telomeres in free living human beings is

TA-65.

I hope this answers your question and again thanks for the interest. This is an amazing field to be part of and an amazing time to be alive!

Dr Dave aka Dr Telomere

WHAT About Improved Athletic performance and TA-65 ?

As far a s TA-65 and performance the input has been “anecdotal”

meaning reported by users but not studied scientifically. It has been extremely positive though. DR Bill Andrews who discovered the human telomerase gene tells of how TA-65 transformed him from a back of the pack ultra-runner to tops in his age group. Two weeks after I started it I dropped a full minute off my 1 mile “interval run” times with no other changes in training or supplementation.

My sister who is in her mid-50’s started to get podium age group finishes 2 months of starting it.

Almost every one notes more energy, but these are all what people notice not what has been actually studied since this is a health and longevity supplement the testing parameters have been limited to that.

In the “Immortality edge” we describe at length the effects of exercise on telomere length and our preferred programs.

Let me also say one thing that is PROVEN and not ANECDOTAL, There is something called overtraining syndrome in athletes. These people lose interest in their sport, start to avhe decreased performance and overall feel poorly as well as having specific signs like poor sleep poor recovery weight loss or gain etc. They definitely have shorter telomeres and this has been documented. In addition their immune systems are far weaker than they should be. On both accounts: protecting the telomeres from shortening and strengthening the immune system there is NO QUESTION TA-65 would be helpful

Can my pet take it!?

TA-65 is not approved for use in animals but i can tell you there are a few people who are doing it. the following animals that are common as “pets” age by telomere loss: humans ( LOL) cats dogs horses. There is one other species of marine mammal that also ages via telomere loss but it is not a common pet. For those of you who follow raw food vegan guru David Wolfe look for an in depth interview on

the topics above soon. All the best, Dr Dave aka Dr Telomere

Do Bioidentical hormones activate telomerase?

While I agree with many of my anti-aging colleagues in that restoration and balancing of human hormone status is essential no one has done a good study on the effects of hormone action and manipulation on the telomeres of living humans that I am aware of. There is A GH study in older Mediterranean men that correlates with GH levels but these were not men who were supplemented; their endogenous GH was high compared to their compatriots and there was no control for other factors.

Ina addition all of the studies mentioned above are on genetically engineered mice and cell cultures not people. The induction of telomerase does not always translate into extension of telomeres.  I have sent every single supplement mentioned above for testing to Sierra Sciences labs as I have mentioned.

None of the above was active at anything resembling typical human dosage. But don’t take my word for it I invite you to do the same.  You can contact Dr Bill Andrews discoverer of the human HTERT gene sequence via Sierra Sciences in Reno.

I think it is important to actually verify what is often stated on the internet to the degree possible and in some cases actually subsidize the testing of agents claimed to do things. I have done so for many supplements directly and of my own volition with the best lab in the country; one that does nothing but test for telomerase activation and its effects on the very hard to induce human fibroblast lines.

As a Board certified anti-aging doc I agree that bioidentical hormones probably do help keep us young and have been prescribing them to men and women since 2000. But it is still a stretch to say that based on a mouse study with receptor engineering that bioidentical hormones reverse aging at least in humans. In truth it is a stretch to claim that anything does so until population based studies on these interventions are done and that will take a long time. In the meantime we do have at least some data

on the subjective and objective effects of telomerase activation via TA-65 on non-engineered human cell lines genetically engineered mice and free living people.

What about hormones? Personally I think they WILL be found to be essential to healthy aging but I doubt they will do anything to extend human lifespan past its own “Hayflick limit” of about 150 years. But do hope human/hormone/telomere length using short telomere testing/ studies are done soon since it will remove yet another argument against their use.

Most recently Elizabeth Blackburn has released a paper on estrogen (it was not specified as “bioidentical by the way”) and telomerase.  She stated in her paper estrogen DECREASES the amount of telomerase expression but appears to affect and slow down cellular aging through another pathway. This might make it viable to slow down aging but it suggests that at least this hormone will not lengthen your lifespan. Still it may improve your healthspan for those of you who don’t care about living longer!

Best, Dr Dave author “THE IMMORTALITY EDGE”

In the following post I give a little bit more personal experience as well as some more questions that are frequently asked about TA-65 telomeres and telomerase

Fountain of youth or fountain of death, my experiences with telomerase activation

Telomere: a chunk of repeating DNA at the end of all chromosomes tied directly to cell health and cell lifespan. It shortens with age and many stresses including sleep deprivation emotional stress physical inactivity diseases and poor dietary choices.

Telomerase: (short for telomere reverse transcriptase) the enzyme that lengthens the telomere, highly active in only a few types of cells

As an expert in the clinical application of telomere biology I get a lot of question from people asking if telomeres and telomerase the enzyme that lengthen them are really the cure for aging or a potential cause for cancer.

The story goes like this. The telomere is a biologic time clock. The “pop culture” application of this and the one we use in our book “The Immortality Edge” states that you can live to 120 years because of telomere length but not much longer. We took some license and called this the Hayflick limit as others before us have even though this is not really what Dr Hayflick

discovered or said.  Bottom line it seems to have stuck and a lot of people say that the Hayflick limit is

the reason we can only live to 120 years. This limit is directly related to the length of the telomere as we now know.

So there you have it a simple easy to understand mechanism of why cells can only divide so many times before they die. Every time the cell reproduces, its one step closer to death and the telomeres are keeping track of the whole process in a sense. Once they get used up and become short, the cell stops dividing and dies. And in recent years the pathways that lead to cell death through aging and disease have been mapped out and ultimately end up shortening the telomere.

The neat thing is that the “telomere theory of aging” includes and helps explain many of the other theories like wear and tear, free radical oxidative damage, the mitochondria theory of aging and many others. As a result we know that all of the things we identify as “unhealthy” including diseases like heart disease and diabetes and conditions like being fat shorten the telomeres and thus shorten our lives.

So what if you can lengthen the telomere or at least slow down the loss?

Well that is the topic of our book “The Immortality Edge”. I along with two other well know anti-aging experts and fellow Telonauts Dr Mike Fossel and Greta Blackburn collaborated to create an “everyman’s guide “to keeping those telomere long. Now most people want to live longer AND a lot healthier and the book is written with that in mind.

Areas of huge interest are drugs and supplements.

Right now the supplement that lengthens telomeres in live free living humans, TA-65, is very expensive and not likely to come down in price soon. Other supplements are often claimed to “turn on telomerase” but when tested in human cell lines at doses that a person could actually take do not work very well or at all.

In addition no other supplement besides TA-65 has human data and this is not likely to change even as people rush new supplements to the market in response to our book and the amazing new findings in telomere biology.

I have personally verified supplements that work and do not work at a world renowned lab that does nothing but test for telomerase activity. That lab is Sierra Sciences and is run by Dr William Andrews a friend of mine and the man who discovered the human telomerase gene. Dr Andrews is responsible for much of what the world knows about telomeres and telomerase. He is one of the truly amazing researchers in the field and in my opinion deserves far more recognition and support than he has gotten for his advances in medicine and of course telomerase biology.

Resveratrol is an example of where much confusion exists.  It is listed as both telomerase activator and telomerase inhibitor.  Indeed before both properties were evaluated by Sierra Sciences the lab that does nothing but test for telomerase. Citations and articles appeared demonstrating both activities which is pretty much impossible. Their results with resveratrol and ones that I trust implicitly show no activity of commercial brands either way.

There are supplements that turn on telomerase or preserve telomere length by other means with enough power to preserve telomere length but slowing down shortening is very different than adding length. TA-65 has been proven to add telomere length in real live human beings.

I mentioned TA-65 was expensive but many people who could afford TA-65 are holding back to see if the concept is real or if there are any side effects.

Is it real?  In the past week and article was released that got a lot of attention. A researcher named Dr DePinho used the telomerase enzyme to make lab mice young again.  At least some of the scientific community reacted with astonishment. While this is an exciting study and certainly appreciated for its contribution, those of us who have been in the field for years are not at all surprised.

This is not the first experiment of its kind.

Almost a decade ago a fellow named Walter Funk who worked with Dr Andrews grafted old human skin onto mice and then turned on telomerase. The skin assumed the characteristics of young skin. Few noticed Funk’s experiment probably because it was “too far ahead of its time”. Last year a human population was discovered with “more active “telomerase.  Telomerase is not active in most of us and that is why we age for the most part. The people who had this more active telomerase lived longer and were healthier as a whole. As a matter of fact the study was done in people over 100 years old.

While I do not discount the importance of studies in genetically altered mice, the people who are taking TA-65 and /or supplements that slow down telomere erosion are doing what amounts to the best kind of experiment. One done in free living humans. A study was published earlier this year in Rejuvenation Research detailing the extreme safety of TA-65 and the objective benefits.

Literally every week a new study comes out in humans linking disease states to shorter telomeres including of course cancer. In addition the converse is true; longer telomeres correlate with less disease longer life and better health.

In what I consider to be the research development of the year Dr Mike West and colleagues (including Walter Funk and Bill Andrews) essentially took differentiated somatic cells typical of aging adults and created the equivalent of an embryonic stem cell line which restored telomerase activity in those adult derived cells to a very early stage.

Simply put they were able to generate very young cells from very old ones. In the process the ethical problems with embryonic stem cells were completely removed. Because of this in the not too distant future you will be able to supply a small amount of your own adult cells from pretty much anywhere on your body and create an embryonic stem cell line with very active “youthful” levels of telomerase and use them to fix what ails you with no compatibility issues since they are “yours” anyway and no ethical issues since you donated them to yourself.

If that is not a huge step to curing aging and the diseases associated with it I don’t know what is.

Another question is cancer. Much of the original work on telomerase was done on cancer since this enzyme telomerase is active in 85% of human cancers and confers “immortality” to them. No telomerase no immortal cancer cell lines.  So scientists continue to try to develop inhibitors to slow down or even help cure cancer.

So turning on telomerase must give you cancer right!?

Wrong. Every study including the ones  done in humans has shown no increase in cancer in people who either are taking a telomerase activator (TA-65) or have the genetics for active telomerase ( very rare!).

The longevity supplements that turn on telomerase are and will be far more “natural” to the way it is turned on in our stem cells and our germ cells 2 lines where we already have active telomerase enzymes. This quite different from the way it is turned on in cancer.

Personally I have no question that telomerase activation is the beginning of the possibility of slowing down or even reversing aging IN HUMANS and I have been on TA-65 for some time now as well as a bunch of other supplements we talk about in the book.

TA-65 has been tested at Sierra Sciences to doses that far exceed anything a person could realistically take and there is no evidence of any toxicity of any kind.

Besides all me, all of my immediate family is on the telomerase activator as well as the supplements we know to support the telomere.  I want my loved ones to live long and be healthy, and I would never give them anything that would harm them.

The bottom line is this: the first real bridge to longer health span and longer life span for PEOPLE is here right now and it’s the topic of our book “The Immortality Edge”. If you want to live longer and healthier for real, this is a great place to start.

I will continue to update you on my experiences and findings. Best, Dr Dave aka Dr Telomere

In the following post there are some specific vitamins and supplements that I have tested for telomerase activation.

Telomere Biology : Anti-aging comes of Age

In an ironic twist of fate the words “anti-aging” which have been so overused up to this point actually

have become meaningful again!

Last September 3 American Scientists Elizabeth Blackburn, Carol Greider and Joe Stostak were awarded the Nobel Prize in medicine for their pioneering work in a “new” field of longevity medicine known as Telomere Biology.

The only problem was their field was not “new”, it began in the 1970’s, and the main focus of telomere

biology was up to this point was involved with cancer prevention.

Nonetheless a huge explosion of research was touched off by this event and it coincided with the general public beginning to understand what telomeres are and what they do .

So what are they and what do they do?

In our individual cells (of which we have trillions depending on who is doing the counting!) there are 46 chromosomes that carry our individual genetic makeup or genetic code if you will. We get half from our mom and half from our dad and together these chromosomes determine what we look like and how we function. Our absolute limits in terms of health intelligence and performance abilities are probably locked in there as well.

On the end of each and every one of those chromosomes is a relatively short segment of “genetic” material that does not code for anything. Its sole function is to determine the absolute lifespan of the cell by a series of “time clock” functions.

Basically every time the cell divides the telomere segment gets shorter and that cell gets closer to death. This is happening in most of the cells of the body.

Poor lifestyle choices like being overweight sleeping to little not dealing with stress in a healthy fashion smoking drinking and even too much exercise of the wrong type can accelerate the loss of these telomere segments and shorten the life of the cells and thus of you and me.

There is good news though in that you can slow down the loss of the telomere segments and you can actually lengthen those segments as well.

Supplements that have been shown in scientific studies to slow down the loss of the telomere segments are:

Fish oil

Vitamin D3

Green Tea and probably its extract

Some forms of Multi-vitamins

There is however a big difference between slowing down the loss of these segments and actually adding length to them

So far the only known substance that actually lengthens telomeres is an expensive supplement known as TA-65. I have personally tested many other substances at a nationally renowned Lab, one fo the few qualified to actually run tests on telomere and the enzyme that lengthens them known as telomerase and I can tell you that as of this writing (October 2010) no other supplements have shown any meaningful activity at a dose that is safe for human consumption.

There are other supplements that make similar claims but I have tested a bunch of them and nothing else works when tested by a reputable lab and subjected to repeat testing.

The other thing you should know about telomeres (and believe me there is so much more to tell you) is that having longer telomeres seems to correlate directly with better health not just longer life!

I have written a book about the topic of longevity and specifically telomerase that is due out in January of 2011. I talk about the topic frequently in my newsletters and blogs. I have lectured to doctors all over the country to educate them on this exciting aspect of longevity. If you would like more information fell free to visit my website.

Take home message is that the word “anti-aging” finally mean just that thanks to the field of telomere biology

All the best in longevity and health! DR Dave Woynarowski M.D.

Dr Dave is a Board certified Internist and Board certified anti-aging physician. He owns and operates his own supplement company TheLongevityEdge.com dedicated to true anti-aging and performance enhancing supplements. Dr Dave still writes his own material and does not use copywriting teams for his newsletters blogs etc. His motto is “Use Mother Nature to Beat Father Time!” He is also co-author of “The Immortality Edge” (Published Dec 2010 by John Wiley and Sons) a book dedicated to teaching everyone about the newest hottest field in anti-aging technology; telomere biology and giving practical proven advice for better health and a longer life that everyone can use RIGHT NOW!

Dr Dave is commonly known as “Dr Telomere”!

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It’s Time for Us to Talk!

So, I have picked next Thursday as the 1st of many telecons to share my time and knowledge with you.

At 7 PM EST March 16th (this Thursday) I’ll be in the hot seat to answer your questions and review some of my favorite topics. To wit we’ll be talking telomeres, telomerase activators and covering a  little basic science so you can stay ahead of the aging curve and ahead of your peers, and way ahead of the competition.

So, block off that time and get your questions ready because I will give until there is nothing left so you get the answers you need!

And of course, there will be at least one special for you as well.  But you need to be live to take advantage of everything I have to offer.

Also, as has ALWAYS been the case in the past, the telecons fill up to capacity long before we do them and ultimately people have to be turned away.

I hate turning people away and you are the last person I want this to happen to- so sign up now and be there on Thursday and we can have that talk both of us always want to have!

Also, a couple of housekeeping issues.

In case you don’t read all the emails (shame on you!) all of the TA-65 from the special  has been sent out and yours should be there any day if its not already!

Next, we are running a special on the newly arrived low dose TA 100 unit 30 count.  It’s a price that is unbeatable so if you want to toe dip, training bra or whatever with TA and do not want to commit to the full dose more expensive product here is a great chance to get it cheap!

This is important: I have gotten a number of disconcerting emails from people asking why they have not received the fish oil they ordered months and months ago. I can only assume you ordered from BioStem Wellness which is using the name DR Dave’s Best to market products.  You will need to take up any issues with them as I have had nothing to do with anything since we “amicably parted company” September 29th 2016. So do not hold me responsible or ask me questions about that business, contact them directly.

My new Omega 3 will be available in about 3 weeks if not sooner.  I will keep you posted but I do not want to keep my people waiting without being sure of my supply chain! I can promise that if you pre order, or order during the launch you will not have to wait 4 months for your product!  Do understand however that the demand for raw materials is very high for even the best most expensive Omega 3 oils and that encapsulation process can take up to 12 weeks so don’t be too hard on the poor people who keep you waiting! It could happen to anyone.

Finally, I will be interviewing yet another telomere god (in this case actually goddess) in about 3 weeks and I also have one or two more telecons planned for you so, stay tuned as always because the momentum is building for me, DR Dave 2.0!

See you Thursday night!!!!

Doc